What is a convective chromatography column?1
Convection chromatography columns are the core components in gel chromatography technology and are usually made of glass tubes or organic glass tubes. It mainly achieves separation based on the difference in distribution coefficients of each component in the sample mixture between the stationary phase and the mobile phase. The key feature of this chromatography method is that the different components of the sample move through the column at different speeds, thereby achieving separation. The diameter of the convection chromatography column does not affect the resolution, but when a large amount of sample is used, the diameter of the column can be increased to meet the needs, especially in preparative gel columns, the diameter is usually greater than 2 cm. In addition, the height of the convection chromatography column has an important impact on the resolution. The resolution is related to the square root of the column height. However, a column that is too high may be blocked due to extrusion deformation, so it is generally not more than 1 meter. In practical applications, convective chromatography columns need to be carefully packed to ensure there is no dead volume, which is critical to achieve efficient separations.
What is a convective chromatography column?_copy1
Convection chromatography columns are the core components in gel chromatography technology and are usually made of glass tubes or organic glass tubes. It mainly achieves separation based on the difference in distribution coefficients of each component in the sample mixture between the stationary phase and the mobile phase. The key feature of this chromatography method is that the different components of the sample move through the column at different speeds, thereby achieving separation. The diameter of the convection chromatography column does not affect the resolution, but when a large amount of sample is used, the diameter of the column can be increased to meet the needs, especially in preparative gel columns, the diameter is usually greater than 2 cm. In addition, the height of the convection chromatography column has an important impact on the resolution. The resolution is related to the square root of the column height. However, a column that is too high may be blocked due to extrusion deformation, so it is generally not more than 1 meter. In practical applications, convective chromatography columns need to be carefully packed to ensure there is no dead volume, which is critical to achieve efficient separations.